Lab Report on Yeast Fermentation Essay Example

Lab Report on Yeast Fermentation Paper The chemical reactions within yeast are facilitated by enzymes; most enzymes optimal temperature range is warm. The warmer the water, the more the enzymes are in their optimal temperature angle and the more efficiently they catalyst chemical reaction; this makes the fermentation proceed more quickly. In this experiment, the effect of variation of temperature on the fermentation of yeast was tested. If temperature affects the fermentation of the yeast, then the rate of yeast fermentation will change with the different levels of temperature. To perform this experiment the following materials were used: 3 empty, clean water bottles, 3 latex balloons, 3 rubber bands, glass measuring cup (1 -cup capacity), teaspoon, tablespoon, all-purpose flour, granulated sugar, 10 degree Celsius water, 29 degree Celsius water, 43 agree Celsius water, and 3 packets of active dry yeast. To start this experiment, each empty, dry water bottle was filled with one 1/4 ounce packet of active dry yeast, 1 teaspoon of granulated sugar, 2 tablespoons of all-purpose flour, and 1 cup of room temperature distilled water. A latex balloon was then secured on the top of each bottle with a rubber band. 3 glass bowls were filled with water of varying temperatures. One water bottle filled with the yeast solution was placed in a glass bowl at 10 degrees Celsius, one placed in a bowl of 29 degrees Celsius, and the other placed in a bowl at 43 degrees Celsius. We will write a custom essay sample on Lab Report on Yeast Fermentation specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Lab Report on Yeast Fermentation specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Lab Report on Yeast Fermentation specifically for you FOR ONLY $16.38 $13.9/page Hire Writer By keeping all glass bowls at their constant initial temperature, observations and results found that temperature does indeed have effect on the fermentation of yeast. The water bottle that was placed in the cold water (10 degrees Celsius) had no change after 20 minutes. The water bottle placed in the room temperature water (29 degrees Celsius) experienced change after 20 minutes; the solution slightly foamed inside the water bottle as the yeast fermented and the balloon inflated to a diameter of 21 h CM. The water bottle placed in the warm water (43 degrees Celsius) experienced change after 20 minutes as well; the solution created very much foam inside the water bottle (about 2 inches above initial solution volume level) and the balloon inflated to a diameter of 30 CM. The picture above was taken with an phone to show visual results gathered by the experiment conducted. In conclusion, the higher the temperature of the water in the glass bowl, the more fermentation the yeast had.

Comparing Japanese and European Serfdom

Comparing Japanese and European Serfdom Although Japan and Europe did not have any direct contact with one another during the medieval and early modern periods, they independently developed very similar class systems, known as feudalism. Feudalism was more than gallant knights and heroic samurai, it was a way of life of extreme inequality, poverty, and violence. What Is Feudalism? The great French historian Marc Bloch defined feudalism as: A subject peasantry; widespread use of the service tenement (i.e. the fief) instead of a salary...; supremacy of a class of specialized warriors; ties of obedience and protection which bind man to man...; [and] fragmentation of authority- leading inevitably to disorder. In other words, peasants or serfs are tied to the land and work for the protection afforded by the landlord plus a portion of the harvest, rather than for money. Warriors dominate society and are bound by codes of obedience and ethics. There is no strong central government; instead, lords of smaller units of land control the warriors and peasants, but these lords owe obedience (at least in theory)  to a distant and relatively weak duke, king or emperor. The Feudal Eras in Japan and Europe Feudalism was well established in Europe by the 800s CE but appeared in Japan only in the 1100s as the Heian period drew to a close and the Kamakura Shogunate rose to power. European feudalism died out with the growth of stronger political states in the 16th century, but Japanese feudalism held on until the  Meiji Restoration  of 1868. Class Hierarchy Feudal Japanese and European societies were built on a system of hereditary classes. The nobles were at the top, followed by warriors, with tenant farmers or serfs below. There was very little social mobility; the children of peasants became peasants, while the children of lords became lords and ladies. (One prominent exception to this rule in Japan was Toyotomi Hideyoshi, born a farmers son, who rose to rule over the country.) In both feudal Japan and Europe, constant warfare made warriors the most important class. Called knights  in Europe and samurai  in Japan, the warriors served local lords. In both cases, the warriors were bound by a code of ethics. Knights were supposed to conform to the concept of chivalry, while samurai were bound by the precepts of bushido, the way of the warrior. Warfare  and Weaponry Both knights and samurai rode horses into battle, used swords, and wore armor. European armor was usually all-metal, made of chain mail or plate metal. Japanese armor included lacquered leather or metal plates with silk or metal bindings. European knights were almost immobilized by their armor, needing help up on to their horses, from where they would simply try to knock their opponents off their mounts. Samurai, in contrast, wore light-weight armor that allowed for quickness and maneuverability, at the cost of providing much less protection. Feudal lords in Europe built stone castles to protect themselves and their vassals in case of attack. Japanese lords, known as  daimyo, also built castles, although Japans castles were made of wood rather than stone. Moral and Legal Frameworks Japanese feudalism was based on the ideas of the Chinese philosopher Kong Qiu or Confucius (551–479 BCE). Confucius stressed morality and filial piety, or respect for elders and other superiors. In Japan, it was the moral duty of the daimyo and samurai to protect the peasants and villagers in their region. In return, the peasants and villagers were duty-bound to honor the warriors and pay taxes to them. European feudalism was based instead on Roman imperial laws and customs, supplemented by Germanic traditions and supported by the authority of the Catholic Church. The relationship between a lord and his vassals was seen as contractual; lords offered payment and protection, in return for which vassals offered complete loyalty. Land Ownership and Economics A key distinguishing factor between the two systems was land ownership. European knights gained land from their lords as payment for their military service; they had direct control of the serfs who worked that land. In contrast, Japanese samurai did not own any land. Instead, the daimyo used a portion of their income from taxing the peasants to provide the samurai a salary, usually paid in rice. Role of Gender   Samurai and knights differed in several other ways, including their gender interactions. Samurai women, for example, were expected to be strong like the men  and to face death without flinching. European women were considered fragile flowers who had to be protected by chivalrous knights. In addition, samurai were supposed to be cultured and artistic, able to compose poetry or write in beautiful calligraphy. Knights were usually illiterate, and would likely have scorned such past-times in favor of hunting or jousting. Philosophy of Death Knights and samurai had very different approaches to death. Knights were bound by Catholic Christian law against suicide  and strove to avoid death. Samurai, on the other hand, had no religious reason to avoid death and would commit suicide in the face of defeat in order to maintain their honor. This ritual suicide is known as seppuku (or harakiri). Conclusion Although feudalism in Japan and Europe has vanished, a few traces remain. Monarchies remain in both Japan and some European nations, though in constitutional or ceremonial forms. Knights and samurai have been relegated to social roles or honorific titles. And socio-economic class divisions remain, though nowhere nearly as extreme.

The piano Research Paper Example | Topics and Well Written Essays - 2000 words

The piano - Research Paper Example This followed the invention of other stringed as well as keyboard instruments leading to the development of the piano as it is today. Between 1790 and 1800, there were major improvements in piano technology and sound due to industrial revolution as suggested by Siepmann, (10). These improvements included the new and high quality steel referred to as the piano wire and also the ability to cast iron frames in a precise manner. There was also the increase of the tonal range of the piano from the initial five octaves to the seven and as it is found in the modern piano. The modern piano has 88 keys with the hammer mechanism which is used to bring out the sound of the strings and then the keys activate the sounding. The strings are held in a cast iron frame which is heavy enough and then they are passed over a kind of bridge to where the strings are tuned. The most crucial element in the sounding of the piano is the soundboard which is made of spruce. Due to the rise of pitch standard in t he modern piano there are few pianos which are left in the market today. In playing the piano, the greater the velocity of a pressed key, the greater the force of hitting the strings with the hammer hence a louder note is produced. The Impact of the Piano in Other Countries The piano has played a major part in the music industry in the many countries. For instance it importantly used in the western jazz, television, classical music and film production. These applications are also done in other parts of the world. Many countries have produced great pianists and composers. This is because the keyboard of the piano gives an easy way of handling complex as well as harmonic interplay, hence a good tool for composition of music. The piano has led to the establishment of piano manufacturing companies such as Yamaha which are situated in different countries thus creating employment. For instance the victory enterprise in Japan is the mostly known leading exporter of the used pianos in the w orld. The music industries in many countries has also grown and developed due to the use of the piano as a composer machine. The piano drama film was produced in New Zealand as it was acted by a pianist. Individuals from different countries have developed their career in music with the inspiration of the piano. The Central Conservatory of Music (CCOM), a music institute in China has a piano department which is a nation al centre facilitating the teaching, performance as well as research. Hundreds of pianists have been trained who are now teaching in other music schools, research institutes in china and have even won international prizes music competition. With the efforts of Chinese pianists combined with that of the foreign pianists from Russia the CCOM has been able to establish a system of piano teaching embracing the Chinese as well as the western philosophies. The piano industry in Japan has tremendously grown due to the world wide demand for the instrument. Thus companies such as Yamaha and Kawai from Japan are major piano producing companies in the world. Piano manufacturing companies have also been established in Korea for instance the Alex Steinbach pianos which is produced by Samick Musical Instruments Company. This has led to the increased involvement in music and piano training schools in Korea. With the increased development of the piano, German entered the field of

The Social Impact of Automobile Essay Example | Topics and Well Written Essays - 1500 words

The Social Impact of Automobile - Essay Example We are popular with possession of the right product, and, at the same time, own our individuality. The car has moved far from being just a tool of convenience or necessity, it has come to life within our minds and our culture. The intricate psychological reasoning for these insecurities is as varied as the individuals themselves. â€Å"Every adult life could be said to be defined by two great love stories. The first – the story of our quest for sexual love – is well known and well-charted. The second – the story of our quest for love from the world – is a more secret and shameful tale. And yet this second love story is no less intense than the first.† (de Botton, 2004). De Botton’s research for possible cures to â€Å"status anxiety† leads to the idea that the admission of ones mortality puts into perspective elements of status, such as careers, possessions, appearance and fame. (Jutkins, 2006). Life experiences that frustrate the fulfillment of people’s basic needs such as independence, competence and interrelationships result in feelings that lead to differing levels of materialistic pursuits. This tendency is especially heightened under the curren t atmosphere of cultural consumerism. To deal with cultural pressures and their effects, people may resort to what psychologist term as compensatory consumption. That is, consuming even more to feel better. â€Å"This is ironic because this additional consumption often stems from the culture of consumerism itself.† (Kasser, Kanner, 2004, p. 16). The market has positioned the most physical of products such as cars, in ways that provide a kind of healing to consumers. Consumers have been drawn into a self-willed daze by an assault of feel-better-about-yourself advertising. The purchase of what we perceive others believe as beautiful possessions enable us to feel significant and worthwhile, at least for a short time. (Davis, 2002). People buy for recognition from family, friends and

Conformity Term Paper Example | Topics and Well Written Essays - 1500 words

Conformity - Term Paper Example As said above, there are several factors that determine the impact of this process. A brief explanation of how some of these factors affect the psychology of a person is provided with apt examples. Conformity is an interesting topic to be discussed in the field of social psychology because the degree to which it affects each person is completely dependent on the external factors. There is also another dimension to it wherein the individual conformity comes into picture. Here, the individual by themselves accept the norms of the group they belong to and follow them. As an individual belonging to any particular group, there are always some norms and cultural rules that need to be followed in order to be in social coherence with the group. The individuals are under immense pressure to follow them so as to be considered a part of that group. In the current world, the generation of youth and peer pressure is forcing more of individuals to succumb to the thoughts of superior groups within their community. They just have to do it to survive that atmosphere. Conformity is more of a group culture inference. Hence the size of the group, their cohesion and the status of each individual in the group play a major part. There are also pundits who believe that conformity is more of a culture oriented subject. They believe that it varies based on different cultures. Asch (1956) has demonstrated an example to prove that conformity is related with culture. He showed that an individual's judgment about a generic issue will be influenced by the norms of the group he belongs to, even though his individual assessment of the same contradicts. All the experiments done by these people have shown that the socio-cultural norms have an impounding effect on the individual conformity exercised. (Asch 1956) Variances of Conformity With the generic meaning of conformity being discussed, the three variances of conformity need to be mentioned. Herber Kelman, famous professor of social ethics had defined three variations of conformity. They are discussed below. Compliance Compliance is a form of conformity where a person, although disagrees about a certain topic within himself, acts to comply with the others in order to please them or to make them feel comfortable. This kind of act can either have a positive effect or a negative on the individual. Basically, a certain line of reference needs to be drawn such that the person can comply with others in order to please them but have to make sure he or she doesn't lose their self esteem in doing so. An example of such activity would be in a professional environment where a worker has to comply with their boss over any kind of issue in order to satisfy them.( Dittes et al 1956) Identification Identification is rather an alternate way of looking at compliance wherein the individual admittedly follows the values and norms of other persons they like. They willingly want to follow them, their roles and be like them. In some cases, it is how they would have been brought up. An example of such a scenario is where an individual follows the footsteps of his/her father and follows his ideals and values. It is because either he/she liked it or it is the way they are brought up. ( Dittes et al 1956) Internalization Internalization is one form which in most cases has the highest probability of causing negative vibes within a person. It is where a

Gene Expression of Mitochondrial Enzymes from Exercise

Gene Expression of Mitochondrial Enzymes from Exercise Induced Change in Gene Expression of Mitochondrial Enzymes as a Result of Intense Exercise Abstract Exercise-induced changes in gene expression of mitochondrial enzymes has become a leading target for sports medicine research. Previous indirect testing of delayed-onset muscle soreness and changes in rotation of motion do not provide a sufficient explanation of the induced changes to the genome (Hubal, et al., 2010). Biomarker testing has allowed for detecting levels of proteins within a sample. Blood and serum levels, taken before and after exercise, have been analyzed and tested for changes in protein activity. Calf-raises show an increase in creatine kinase (CK) and aldolase (ALD) activities (Kanda, et al., 2014). MicroRNA arrays along with telomere extension mRNA arrays and quantitative real-time PCR on RNA taken from white blood cells have shown to down-regulate telomeric repeat binding factor 2 (Chilton, et al., 2014). Biochemical testing at the genomic level will provide a better understanding of the long-term effects of intense exercise. Knowing these high-intensity induced gene expressions in mitochondrial DNA aids in knowing what causes diseases such as Rhabdomyolysis. Introduction Centuries ago staying physically fit was accentual to stay alive. Those that were not fit were not able to kill prey and therefore would be less likely to survive. In today’s world, being physically fit is not a necessity, but rather something that many people strive for to live a healthy lifestyle. Living an active life lowers the chance of many diseases, such as kidney and Alzheimer’s, and cancers, such as colon and breast. It is therefore crucial to understand the biochemistry behind exercise as a helpful preventative measure for health problems. When exercising, the body is put through tasks that disrupt homeostasis. The body wants to eliminate wide spread deviants of homeostasis. However, after and during exercise the body needs more oxygen and energy to be able to complete the tasks one is putting on the body. These demands, the increase in affinity for oxygen and energy, require metabolic responses that disrupt homeostasis. To test these metabolic responses, many scientists use biomarker testing on whole blood and serum samples rather than pieces of skeletal muscle. Biomarkers are used to measure the presence of a physiological state. These markers have biological properties that measure the blood and serum. There are many different changes in the mitochondrial genome during and immediately following exercise. This paper will focus on an overview of some endurance training biomarkers, but will mainly focus on high intensity exercise and the induced gene expression in the mitochondrial genome. It is important to study the effects of exercise on gene expression to know at what levels of various genes, like lactate dehydrogenase, aldose, etc., come dangerous to human health as to prevention diseases such as Rhadomyolysis (Heled, et al., 2005). Proliferator-activated Receptors association with Exercise-Induced Mitochondrial Biogenesis Transcriptional cofactors peroxisome proliferator-activated receptors (PGC) regulate gene expression (Lin, et al., 2005). PGC-1a regulates gene expression in the mitochondrial genome. This coactivator interacts with other proteins to regulate contraction. Overexpression of PGC-1a results in an increase in mitochondrial gene function (Lin, et al., 2002). Calcium/Calmodulin-dependent kinase 2 (CaMKII), AMP-activated protein kinase (AMPK), and nitrogen-activated protein kinases (MAPKs) all have important signaling that help regulate PGC-1a (Hawley, et al., 2010). Calcium is important for energy due to its role in the sarcoplasmic reticulum. Contractions of skeletal muscle are dependent on high levels of calcium. The calcium then binds to troponin, which moves the myosin fibers resulting in muscle contractions. Calcium also plays a major role in calcium-calmodulin-dependent kinases. Calcium/Calmodulin-dependent kinase 2 (CaMKII) activation increases the transport of glucose in skeletal muscle (Rose, A. and Hargreaves, M., 2003). AMPK and MAPks activate PGC-1a, Figure 1, by phosphorylating transcription factors myocyte enhancer factor 2 and ATF-2, respectively (McGee and Hargreaves, 2010). Figure 1. Schematic of the Major Signaling Pathways Involved in the Control of Skeletal Muscle Hypertrophy and Mitochondrial Biogenesis (Hawley, et al., 2010). The right side of this figure shows the correlation of the AMPK, CAMK, SIRT1, MAPK pathways that effect PGC-1a which regulates mitochondrial biogenesis. Another inhibitor of PGC-1a is a deactelyase silent mating type-information regulation 2 homolg 1 (SIRT1) (HIgashida, et al., 2013). Since SIRT1 is NADà ¢Ã‚ Ã‚ º-dependent, Figure 1, changes in concentration of NADà ¢Ã‚ Ã‚ º change the SIRT1 activity in the cell (Gurd, 2011). Many studies have shown that the protein content of SIRT1 and the activity are independent, and that because of that it is thought that the SIRT1 activity is what subsidizes to PGC-1a activity (Gurd 2011). Test For Correlation between SIRT1 and PGC-1a. The following experiment was performed by Chabi and coworkers to examine SIRT1’s role in muscle during muscle use (Chabi, et al., 2009). Rats were placed into a control group and a running group. The running group were able to run on a loaded wheel. Weight was added to the wheel during weeks one through four, but maintained a 200 g weight from weeks five through eight. The plantaris (PL) and soleus (SOL) muscles were taken as well as the tibialis anterior (TA) and extensor digitorum longus (EDL) for analysis. Once the proteins were extracted, the SIRT1 activity and deacetylase was found by fluorescence. A cycling assay was used to determine the NAD nucleotides. Immunoblotting was done to test expression of PGC-1a, cytochrome c, SIRT1 and GAPDH. SIRT1 expression showed to be the highest in the liver and slow-twitch muscle while PGC-1a immunoreactivity was highest in the heart muscle. This experiment did not show a correlation between SIRT1 and PGC-1a expression, like the scientists hoped, but it did bring up the question if SIRT1 activity is altered by acute exercise instead of high-intensity. Eccentric Exercise and Muscle Damage Markers The following was performed by Kazue Kanda and coworkers to see if eccentric exercise affects muscle damage markers (Kanda, et al., 2014). Participates in this study each performed right calf-raises on a force plate to add 0.5 Hz to each lift. With 3 min for rest, forty repetitions for 10 sets were completed with half of their weight along with the added force. Immediately following these muscle contractions, the medial and lateral gastrocnemius and soleus were measured for tenderness using a FP meter. The meter rated based on a visual analogue scale from no pain to extremely sore. The ankle was tested for range of motion (ROM) along the dosiflexion position (-20 ) to the plantar flexion position (15). Both of tenderness of the calf and the ROM was tested at 24 h increments until 168 h after the repetitive muscle contractions. After 72 h the tenderness of the right calf increased significantly (p Subsequently, samples from the blood and urine were taken both before and at various times after the exercise. Various proteins, creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST), aldolase (ALD) and alanine aminotransferase (ALT) were tested from the serum. An Enzyme-linked immunosorbent assay (ELISA) was used to test for fatty acid-binding proteins (FABP). The activities of CK and AST increased significantly post 72 h. The activities of ALT and ALD also increased for each sample, however, it was not significant. LDH activity had a significant increase at 96 h after exercise, but not really before. The results for ALD did correlate with the tenderness of the medial gastrocnemius at the 72 h mark and could suggest that ALD might be a better muscle damage indicator because the other proteins tested did not correlate with tenderness. The ELISA testing on FABP resulted in no detectable changes. This experiment used two methods of testing muscle damage and had correlated results for ALD. Exercise-Induced biochemical changes in Quadriceps and Gastrocnemius in Mice The following was performed by L. Toti and coworkers to see the changes in blood lactate as well as mitochondrial enzymes as a result of two different exercises; intense activity with recovery periods and moderate activity with no recovery periods (Toti, et al., 2013). Immunoblotting and immunohistochemistry were used to assess the two muscle groups, the quadriceps and gastrocnemius, to see expression of enzymes correlated with oxidative metabolism. Mice were divided into three different groups based on similar maximal velocities. Mice with higher maximal velocities partaked in the intense activity: running at 90% for 2 min followed by 1 min of recovery. The mice with lower mamximal velocities partaked in continuous running at 60% velocity. Data was collected for 40 sessions, where each mouse ran a distance of 1000 meters. Blood samples were taken at sessions 1, 20 and 40. Blood lactate was tested and showed a decrease in both groups by session 40, with the higher-velocity group measuring significantly lower than the lower-velocity group. Immunochemistry testing resulted in an increase in response for the high-velocity mice for both the quadriceps and the gastrocnemius. This experiment showed that high-intensity training impacted the biochemistry more so than the low-velocity training. MicroRNA Expression and Telomere-Associated Genes After Acute Exercise The following was performed by W. Chilton and coworkers to see mechanisms the correlation between white blood cell (WBC) telomere length and exercise (Chiltion, et al., 2014). Participants in this study ran on a treadmill for 30 min at 80% of maximum oxygen uptake. Blood samples were taken before and immediately following the running as well as an hour post-running. MicroRNA expression arrays that could measure a whole genome, were used on the samples. TERT mRNA expression levels were then tested by qPCR. Telomerase reverse transcriptase (TERT) mRNA and Sirtuin-6 (SIRT6) were two of the genes tested. This experiment was able to show supporting evidence that the transcriptional regulation of key telomeric genes can be affected by exercise. TERT mRNA was upregulated as well as the SIRT6. The qPCR testing on TERT and SIRT6 showed the increase in binding miRNA. Chilton did express that the increases in both the SIRT6 and the TERT mRNAs could have been upregulations from the extra-telomeric pathways instead of just the telomeric roles since there was no definitive way to differentiate between the two in this experiment. It is important to understand exercise’s effects on telomeres and its corresponding proteins to gain an insight on how physical health improves telomere homeostasis, keeping the telomeres from getting too short and the cell dying. Chemokine Polymorphisms Association with Skeletal Muscle Damage The following experiment was performed by M. Hubal and coworkers to see if chemokine ligand 2 (CCL2) and chemokine receptor 2 (CCR 2) are associated with biomarkers after exercising (Hubal, et al., 2010). CCL2 is important because it recruits necessary items, such as memory T cells, dendritic cells, and monocytes, to inflammation sites in injured tissue. CCR2, the receptor molecule for CCL2, mediates with calcium mobilization. It is suggested that CCL2 and CCR3 play major roles in the repair of skeletal muscle damage. Participants performed two, 25 contractions, sets of elbow flexor muscle contractions in the non-dominant arm. It was crucial that the participants had constant maximal effort and stayed hydrated the 10 days following the exercise. Blood samples were taken and sent for genotyping. Single nucleotide polymorphisms (SNPs) known to influence the level of CCL2 proteins were analyzed using PCR. There were four SNPs from CCL2 and three from CCR2 that should high phenotype associations. The minor allele found in the SNPs was correlated with an increase in damage. This study was able to show that variations of the CCL2 and CCR2 genes are related to muscle damage markers caused by exercise. Molecular and Metabolic Changes of High-intensity Interval Training The following experiment was performed by J. Little and coworkers to assess the molecular and metabolic changes of high-intensity interval training (Little, et al., 2010). Participants performed six cycling training sessions over the course of two weeks. Each session consisted of approximately 30 min of high intensity intervals. By the last sessions, the subjects were completing 12 intervals of 60 s high-intensity cycling followed by 75 s low intensity for recovery. Biopsies from the leg were taken before and after the two week training. The muscle lysates were taken for Western blotting and enzyme activity testing. Western blotting was used to test for glucose transporter type 4 (GLUT4), PGC-1a, and SIRT1while the mitochondrial enzyme activity was tested on cytochrome c oxidase (COX). The subjects improved in both time and power, about 10%, for cycling during the two week training session. COX activity increased by 29%. PGC-1a increased by approximately 24%, however, the protein itself was did not have any genetic changes. GLUT4 content increased by 119%, while SIRT1 increased by approximately 56%. This experiment showed some changes, due to exercise, in regulators that are important in mitochondrial biogenesis. Conclusion There are many induced changes in the mitochondrial genome during and after exercise. These changes occur to help maintain cell homeostasis while the body is being put through stress during intense exercise. Blood and serum samples along with tissue extractions have provided a way to examine these changes and see how one is correlated with another (Figure 1). PGC-1a helps muscle contractions by regulating gene expression in mitochondria biogenesis. However, PGC-1a has many cofactors helping it. CaMKII, AMPK, and MAPKs all help in providing energy to the skeletal muscles by impacting the activation of PGC-1a. Although SIRT1 deactelyation inhibits PGC-1a, it still impacts the biochemistry of the body during workouts due to deactelyation. LDH activity was shown to increase hours after high-intensity exercise because LDH it is released as pyruvate is converted into lactate. ALD, which converts sugar into energy, was found to be a good indicator because in the calf-raises experiment the results from the biomarker testing as well as the tenderness testing correlated with each other. CCL2 and CCR2 were found to have changes in SNPs that corresponded to the high-intensity exercise and most likely aide in the recruiting of the memory T cells and dendritic cells to the injured tissue. High-intensity exercise, without time for recovery, would keep the body maintained at a stressful state of trying to bring it back to homeostasis. As proven in the calf-raise experiment, LDH levels decreased as the participants were able to come accustom to the exercises. The bodies were no longer in shock. If the bodies were did not become accustom, or the body was not given any time for recovery, the chances of obtaining diseases such as Rhabdomyolysis increases. Future experiments need to focus on what levels of these regulators will become dangerous. Research should be down to further understand the relationship between SIRT1 and PGC-1a. References Chabi, B., Adhihetty, P.J., O’Leary, M.F., Menzies, K.J., and Hood, D. (2009) Relationship between sirt1 expression and mitochondrial proteins during conditions of chronic muscle use and diuse. J. Appl. Physiol. 107(6):1730-1735. Chilton, W., Marques, F., West, J., Kannourakis, G., Berzins, S., O’Briend, B., and Charchar, F. (2014) Acute Exercise Leads to Regulation of Telomere-Associated Genes and MicroRNA Expression in Immune Cells. PLoS ONE. 9, 1-13. Gurd, B. (2011) Deacetylation of PGC-1a by SIRT1: importance for skeletal muscle function and exercise-induced mitochondrial biogenesis. App. Physiol. Nutr. Metab. 36:589-597. Hawley, J., Hargreaves, M., Joyner, M., and Zierath, J. (2010) Integrative Biology of Exercise. Cell 159, 738-749. Heled., Y., Zarian., A., Moran., D., and Hadad, E. (2005) Exercise induced rhabdomyolysis – characteristics, mechanisms and treatment. Harefuah 144(1):34-8. Hubal, M., Devaney, J., Hoffman, E., Zambraski, E., Gordish-Dressman, H., Kerns, A., Larking, J., Adham, K., Patel, R., and Clarkson, P. (2010) CCL2 and CCR2 polymorphisms are associated with markers of exercise-induced skeletal muscle damage. J of App Physicol 108(6), 1651-1658. Kanda, K., Sugama, K., Sakuma, J., Kawakami, Y., and Suzuki, K. (2014) Evaluation of serum leaking enzymes and investigation into new biomarkers for exercise-induced muscle damage Exerc Immunol Rev. 20, 39-54. Lin, J., Wu, H., Tarr, P., Zhang, C., Wu, Z., Boss, O., Michael, L., Puigserver, P., Isotani, E., Olson, E., Lowell, B., Bassel-Duby, R., and Spiegelman, B. (2002). Transcriptional co-activator PGC-1 alpha drives the formation of slow-twitch muscle fibres. Nature 418, 797-801. Lin, J., Handschin, C., and Spiegelman, B. (2005). Metabolic control through the PGC-1 family of transcription coactivators. Cell. Metab. 1, 361-370. Little, J.P., Safdar, A., Wilkin, G.P., Tarnopolsky, M.A., and Gibala, M.J. (2010) A practical model of low-volume high intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. J. Physiol. 588(6):1011-1022. McGee, S., Hargreaves, M. (2010). AMPK-mediated regulation of transcription in skeletal muscle. Clin. Sci. 118, 258-263. Rose, A.J., and Hargreaves, M. (2003). Exercise increases -calmodulin-dependent protein kinase II activity in human skeletal muscle. J. Physiol. 265, E380-E391. Toti, L., Bartalucci, A., Ferrucci, M., Fulceri, F., Lazzeri, G., Lenzi, P., Soldani, P., Gobbi, P., La Torre, A., and Gesi, M. (2013) High-intensity exercise training induces morphological and biochemical changes in skeletal muscles. Biol Sport 30(4), 301-309. Alissa Christian

Organizational Behavior Essay -- essays research papers

Organizational Behavior: A Case of Effective Management   Ã‚  Ã‚  Ã‚  Ã‚   â€Å"Organizational structure is the formal system of task and reporting relationships that controls, coordinates, and motivates employees so that they cooperate and work together to achieve the organization’s goals†. (George et al, 2002). The importance of structure can’t be stressed enough. Having an organizational structure ensures that each member of the organization has the correct goals of their particular department set. A good structure will give people the motivation needed to achieve those goals, and enhance their performance. As discussed in our text, in order to encourage work, cooperation, and the drive needed from employees, an organization should have some sort of organizational structure. Whether a functional structure, a divisional structure, a network structure, or even a matrix structure, every corporation must have one type to achieve the directives set forth by their mission statement. A strong structure essentially changes the way people work, and the results of their work ethics will produce quality work. If we look at the Aberdeen plant, we see what I believe to be, a functional structure. This structure, that Lancaster as developed, has proven to be quite a success. Each employee is a member of a group, and each group has a leader that has been chosen by the team members. If we try to put Aberdeen in an organizational chart, it will look something like this: Then, from the team leaders, come the members of each group, and information is shared between everyone within the hierarchy. This is what forms the trust needed, and helps coordinate people, and encourages motivation. At Green River, this type of structure can be implemented, but not without doing away with the union. The union, in essence, has its own hierarchy within the union itself. Here, at Green river, I can see more of a divisional structure with a concentration on the product structure, or quite possibly a market structure. Green River is part of Alkali Chemicals, which is a division of FMC that supplied to many different areas of the market. Its goal was to produce sodium-based chemicals to the detergent industry, the glass industry, and large commercial chemical plants (Clawson et al, 2003). This type of structure does not allow for much group interactivity as a formal structure ... ...in the organizational goals. Having done this, the organization can build a strong culture, and increase the performance of its employees. At Green River, the culture is built on an interactive management style from Dailey. He has built high levels of trust with his employees, and he is always willing to share information with them. This type of culture has proven to be successful in his plant. If he wants to improve the culture, then maybe he can try to do as Aberdeen and have employee picnics and such, and having them bring their families. Or, Dailey can incorporate a new code of ethics. Make the union members responsible for their own actions, and promote the fact that he will always be there to offer support if anyone has problems to deal with, professionally or personally. Promote the company as being a family unit. This can build a strong relationship with both organizations†¦the union organization and Green River management. References Clawson, J. (2003). Custom business resources. Custom ed.: Prentice Hall. George, Jennifer M., & Jones, Gareth R. (). Organizational behavior. 3rd ed. Upper Saddle River, New: Prentice Hall.  Ã‚  Ã‚  Ã‚  Ã‚